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cd2 antibody, anti-human, reafinity  (Miltenyi Biotec)


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    Structured Review

    Miltenyi Biotec cd2 antibody, anti-human, reafinity
    Cd2 Antibody, Anti Human, Reafinity, supplied by Miltenyi Biotec, used in various techniques. Bioz Stars score: 93/100, based on 4 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/cd2/CD2+Antibody%2C+anti-human%2C+REAfinity/custom%40130-119-507%4042502392
    Average 93 stars, based on 4 article reviews
    cd2 antibody, anti-human, reafinity - by Bioz Stars, 2026-10
    93/100 stars

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    Related Articles

    Staining:

    Article Title: Ångström Resolution with Flow Immunofluorescence Localization Microscopy (FILM)
    Article Snippet: .. α-tubulin (REA1136 REAfinityTM, h, APC, #130-119-541, Miltenyi Biotec), CD2 (REA972 REAfinityTM, h, APC, #130-116-150, Miltenyi Biotec), CD20 (REA780 REAfinityTM, h, APC, #130-111-339, Miltenyi Biotec), CD28 (REA612 REAfinityTM, h, APC, #130-118-343, Miltenyi Biotec), CD45 (REA1023 REAfinityTM, h/nhp, APC, #130-117-191, Miltenyi Biotec), CD54/ICAM-1 (REA266 REAfinityTM, h, APC, #130-120-711, Miltenyi Biotec), CD81 (REA513 REAfinityTM, h, APC, #130-119-787, Miltenyi Biotec), CD107a/LAMP1 (REA792 REAfinityTM, h, APC, #130-111-847, Miltenyi Biotec), IgD (REA740 REAfinityTM, h, APC, #130-110-644, Miltenyi Biotec), IgM (REAL689 REAdye_leaseTM, h, PE, #130-125-786, Miltenyi Biotec), Pan-Cytokeratin (REA1141 REAfinityTM, h, APC, #130-120-096, Miltenyi Biotec), β-catenin (REA480 REAfinityTM, h, APC, #130-124-444, Miltenyi Biotec), TCRα/β (REA652 REAfinityTM, h, APC, #130-113-535, Miltenyi Biotec), TOM22 (REA1185 REAfinityTM, APC, h/ms, #130-122-079, Miltenyi Biotec), DAPI Staining Solution (#130-111-570, Miltenyi Biotec), and ATTO 643 phalloidin (ATT-AD643-81, ATTO-TEC). ..

    Labeling:

    Article Title: Markers of immunosenescence in CMV seropositive healthy elderly adults
    Article Snippet: The PBMC of each study participant were thawed and washed in AIM-V medium (Gibco, ThermoFisher) to be used in all experiments. (i) Monocyte and natural killer subpopulations were evaluated in ex vivo . .. A volume of 1 × 10 6 cells in 50 ul of PBS labeled with one of the following monoclonal antibody panels from Biolegend: Monocytes : with FITC anti-CD14 (M5E2), PE/Cy5 anti-CD16 (3G8), PE/Dazzle594 anti-HLA-DR. Natural killer : Pacific blueTM anti-CD3, FITC anti-CD56 (5.1H11), APC anti-CD57 (HNK-1), APC/FireTM750 anti-KLRG1(SA231A2), PECy7 anti-NKp30 (P30-15), PE anti -NKG2D (1D11). (ii) One million PBMCs were seeded in 96-well flat-bottom dishes for 72 h with a mixture of beads coupled to antibodies against CD3, CD28, and CD2 (Miltenyi Biotec) in a 2:1 ratio (PBMCs: beads) grown in AIM-V medium (Thermo Fisher Scientific), This stimulation ensures sufficient activation and upregulation of key markers, such as PD1, which reflect the activation status and differentiation potential of the T cells ( ). .. After incubation, the cells were washed with PBS and labeled with of the following monoclonal antibody panels from Biolegend: Pacific blueTM anti-CD3, Brillant violet 510TM anti-CD4 + (SK3), PE/Dazzle594 anti-CD8 (SK1), FITC anti-CD45RO (UCHL1), Alexafluor700 anti-CD62L (DREG-5b), APC anti-CD57 (HNK-1) y APC/FireTM750 anti-KLRG1(SA231A2).

    Article Title: Markers of immunosenescence in CMV seropositive healthy elderly adults.
    Article Snippet: The PBMC of each study participant were thawed and washed in AIM-V medium (Gibco, ThermoFisher) to be used in all experiments. (i) Monocyte and natural killer subpopulations were evaluated in ex vivo. .. A volume of 1 × 106 cells in 50 ul of PBS labeled with one of the following monoclonal antibody panels from Biolegend: Monocytes: with FITC anti-CD14 (M5E2), PE/ Cy5 anti-CD16 (3G8), PE/Dazzle594 anti-HLA-DR. Natural killer: Pacific blueTM anti-CD3, FITC anti-CD56 (5.1H11), APC anti-CD57 (HNK-1), APC/FireTM750 anti-KLRG1(SA231A2), PECy7 anti-NKp30 (P30-15), PE anti -NKG2D (1D11). (ii) One million PBMCs were seeded in 96-well flat-bottom dishes for 72 h with a mixture of beads coupled to antibodies against CD3, CD28, and CD2 (Miltenyi Biotec) in a 2:1 ratio (PBMCs: beads) grown in AIM-V medium (Thermo Fisher Scientific), This stimulation ensures sufficient activation and upregulation of key markers, such as PD1, which reflect the activation status and differentiation potential of the T cells (Cossarizza et al., 2022). .. After incubation, the cells were washed with PBS and labeled with of the following monoclonal antibody panels from Biolegend: Pacific blueTM anti-CD3, Brillant violet 510TM antiCD4+ (SK3), PE/Dazzle594 anti-CD8 (SK1), FITC anti-CD45RO Frontiers in Aging frontiersin.org02 (UCHL1), Alexafluor700 anti-CD62L (DREG-5b), APC anti-CD57 (HNK-1) y APC/FireTM750 anti-KLRG1(SA231A2).

    Activation Assay:

    Article Title: Markers of immunosenescence in CMV seropositive healthy elderly adults
    Article Snippet: The PBMC of each study participant were thawed and washed in AIM-V medium (Gibco, ThermoFisher) to be used in all experiments. (i) Monocyte and natural killer subpopulations were evaluated in ex vivo . .. A volume of 1 × 10 6 cells in 50 ul of PBS labeled with one of the following monoclonal antibody panels from Biolegend: Monocytes : with FITC anti-CD14 (M5E2), PE/Cy5 anti-CD16 (3G8), PE/Dazzle594 anti-HLA-DR. Natural killer : Pacific blueTM anti-CD3, FITC anti-CD56 (5.1H11), APC anti-CD57 (HNK-1), APC/FireTM750 anti-KLRG1(SA231A2), PECy7 anti-NKp30 (P30-15), PE anti -NKG2D (1D11). (ii) One million PBMCs were seeded in 96-well flat-bottom dishes for 72 h with a mixture of beads coupled to antibodies against CD3, CD28, and CD2 (Miltenyi Biotec) in a 2:1 ratio (PBMCs: beads) grown in AIM-V medium (Thermo Fisher Scientific), This stimulation ensures sufficient activation and upregulation of key markers, such as PD1, which reflect the activation status and differentiation potential of the T cells ( ). .. After incubation, the cells were washed with PBS and labeled with of the following monoclonal antibody panels from Biolegend: Pacific blueTM anti-CD3, Brillant violet 510TM anti-CD4 + (SK3), PE/Dazzle594 anti-CD8 (SK1), FITC anti-CD45RO (UCHL1), Alexafluor700 anti-CD62L (DREG-5b), APC anti-CD57 (HNK-1) y APC/FireTM750 anti-KLRG1(SA231A2).

    Article Title: Markers of immunosenescence in CMV seropositive healthy elderly adults.
    Article Snippet: The PBMC of each study participant were thawed and washed in AIM-V medium (Gibco, ThermoFisher) to be used in all experiments. (i) Monocyte and natural killer subpopulations were evaluated in ex vivo. .. A volume of 1 × 106 cells in 50 ul of PBS labeled with one of the following monoclonal antibody panels from Biolegend: Monocytes: with FITC anti-CD14 (M5E2), PE/ Cy5 anti-CD16 (3G8), PE/Dazzle594 anti-HLA-DR. Natural killer: Pacific blueTM anti-CD3, FITC anti-CD56 (5.1H11), APC anti-CD57 (HNK-1), APC/FireTM750 anti-KLRG1(SA231A2), PECy7 anti-NKp30 (P30-15), PE anti -NKG2D (1D11). (ii) One million PBMCs were seeded in 96-well flat-bottom dishes for 72 h with a mixture of beads coupled to antibodies against CD3, CD28, and CD2 (Miltenyi Biotec) in a 2:1 ratio (PBMCs: beads) grown in AIM-V medium (Thermo Fisher Scientific), This stimulation ensures sufficient activation and upregulation of key markers, such as PD1, which reflect the activation status and differentiation potential of the T cells (Cossarizza et al., 2022). .. After incubation, the cells were washed with PBS and labeled with of the following monoclonal antibody panels from Biolegend: Pacific blueTM anti-CD3, Brillant violet 510TM antiCD4+ (SK3), PE/Dazzle594 anti-CD8 (SK1), FITC anti-CD45RO Frontiers in Aging frontiersin.org02 (UCHL1), Alexafluor700 anti-CD62L (DREG-5b), APC anti-CD57 (HNK-1) y APC/FireTM750 anti-KLRG1(SA231A2).

    Article Title: Substituted hydroxystilbene compounds and derivatives synthesis and uses thereof
    Article Snippet: .. Key Assay Parameters Human CD4-postive T-cells isolated from buffy coats and kept at −150 degree C. until day of assay Stimulation Beads coaled with antibodies to CD2, CD3 and CD28 (T-cell activation/expansion kit Miltenyi Biotec cat no 130-091- 441) One bead/cell. .. Effect Inhibition of IL-4 release from T-cells 0% effect of Bead stimulation, 0.1% DMSO test compound 100% effect of Unstimulated cells, 0.1% DMSO test compound Reference compound Betamethasone (CAS: 378-44-9) Incubation time 48 h Capacity, run-time The assay takes 3 days to perform. and requirements Method Description CD4+ T-Cell Isolation: Peripheral blood mononuclear cells (PBMC) are isolated from fresh human buffy coats by centrifugation on Lymphoprep® following instructions from SebmateTM (Stemcell).

    Isolation:

    Article Title: Aspergillus fumigatus extract modulates human eosinophils via NOD2 and oxidative stress.
    Article Snippet: .. Isolation of eosinophils from human peripheral blood We used a negative selection method with biotin-conjugated antibodies for CD2, CD14, CD16, CD19, CD56, CD123, and CD235a and anti-biotin microbeads to isolate eosinophils from the peripheral blood of healthy volunteers, according to the manufacturer's protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). .. This study was approved by the Institutional Review Board of Keio University School of Medicine (20200100, 20090009) and National Defense Medical College (4515).

    Article Title: Substituted hydroxystilbene compounds and derivatives synthesis and uses thereof
    Article Snippet: .. Key Assay Parameters Human CD4-postive T-cells isolated from buffy coats and kept at −150 degree C. until day of assay Stimulation Beads coaled with antibodies to CD2, CD3 and CD28 (T-cell activation/expansion kit Miltenyi Biotec cat no 130-091- 441) One bead/cell. .. Effect Inhibition of IL-4 release from T-cells 0% effect of Bead stimulation, 0.1% DMSO test compound 100% effect of Unstimulated cells, 0.1% DMSO test compound Reference compound Betamethasone (CAS: 378-44-9) Incubation time 48 h Capacity, run-time The assay takes 3 days to perform. and requirements Method Description CD4+ T-Cell Isolation: Peripheral blood mononuclear cells (PBMC) are isolated from fresh human buffy coats by centrifugation on Lymphoprep® following instructions from SebmateTM (Stemcell).

    Selection:

    Article Title: Aspergillus fumigatus extract modulates human eosinophils via NOD2 and oxidative stress.
    Article Snippet: .. Isolation of eosinophils from human peripheral blood We used a negative selection method with biotin-conjugated antibodies for CD2, CD14, CD16, CD19, CD56, CD123, and CD235a and anti-biotin microbeads to isolate eosinophils from the peripheral blood of healthy volunteers, according to the manufacturer's protocol (Miltenyi Biotec, Bergisch Gladbach, Germany). .. This study was approved by the Institutional Review Board of Keio University School of Medicine (20200100, 20090009) and National Defense Medical College (4515).



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